The alignment is done after the lengths and auto margins are applied, meaning that, if in a Flexbox layout there is at least one flexible element, with flex-grow different from 0, it will have no effect as there won't be any available space

/* Positional alignment */
justify-content: center;     /* Pack items around the center */
justify-content: start;      /* Pack items from the start */
justify-content: end;        /* Pack items from the end */
justify-content: flex-start; /* Pack flex items from the start */
justify-content: flex-end;   /* Pack flex items from the end */
justify-content: left;       /* Pack items from the left */
justify-content: right;      /* Pack items from the right */

/* Baseline alignment */
/* justify-content does not take baseline values */

/* Normal alignment */
justify-content: normal;

/* Distributed alignment */
justify-content: space-between; /* Distribute items evenly
                                   The first item is flush with the start,
                                   the last is flush with the end */
justify-content: space-around;  /* Distribute items evenly
                                   Items have a half-size space
                                   on either end */
justify-content: space-evenly;  /* Distribute items evenly
                                   Items have equal space around them */
justify-content: stretch;       /* Distribute items evenly
                                   Stretch 'auto'-sized items to fit
                                   the container */

/* Overflow alignment */
justify-content: safe center;
justify-content: unsafe center;

/* Global values */
justify-content: inherit;
justify-content: initial;
justify-content: unset;